X
  • 货号
  • CT-001
  • 规格
  • 2L/套

产品简介

本产品用于从单个核细胞(MNC)在体外高效扩增成NK细胞,具有扩增效率高,目的细胞百分比高的特点,起始单个核细胞经过14天培养,总细胞数可以扩增约50-100倍,NK细胞数可以扩增500倍以上,且纯度可达75%-95%(由于个体差异,有的样品诱导后NK细胞纯度低于75%或高于95%)。

产品优势

  成分明确,无异源成分

  无菌,无支原体,低内毒素

  纯因子法培养

  同时适用于脐血与外周血(更适配脐血)

  14天培养后,细胞活率在90%以上

  纯度较高,14天培养后大多样本的纯度可达75-95%


详情介绍 参考文献


  NK细胞形态


  NK2.0细胞生长曲线


 14天扩增后流式表型

操作链接:

https://www.bilibili.com/video/BV1BjsUeXE2w/

[1] Wu J, Gao F X, Wang C, et al. IL-6 and IL8 secreted by tumour cells impair the function of NK cells via the STAT3 pathway in oesophageal squamous cell carcinoma[J]. Journal of Experimental and Clinical Cancer Research, 2019, 38(1): 321.

[2] Han B, He J, Chen Q, et al. ELFN1-AS1 promotes GDF15-mediated immune escape of colorectal cancer from NK cells by facilitating GCN5 and SND1 association[J]. Discover Oncology, 2023, 14(1): 56.

[3] Kuang Y L, Liang S M, Kang J, et al. Inhibition of miR-20a by pterostilbene facilitates prostate cancer cells killed by NK cells via up-regulation of NKG2D ligands and TGF-β1down-regulation[J]. Heliyon, 2023, 9(4): e14957.



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